(226) Tissue Culture of Vitis sp. for Protoplast Isolation
نویسندگان
چکیده
منابع مشابه
Isolation of Adipose Tissue Stem Cells with Organ Culture Method
Background Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of MSCs. Also, it is accompanied with the increased risk of culture contaminations because of several handling steps.In this...
متن کاملObservations and Suggestions for Improving Somatic Hybridization by Plant Protoplast Isolation, Fusion, and Culture
De (Triticum aestivum): In vitro production and utilization of doubled haploids, p. 101–124. In: Y.P.S. Bajaj (ed.). Biotechnology in agriculture and forestry. vol. 12: Haploids in crop improvement I. Springer-Verlag, Berlin. Powell, W., P.D.S. Caligari, and J.M. Dunwell. 1986. Field performance of lines derived from haploid and diploid tissues of Hordeum vulgare. Theor. Applied Genet. 72:458–4...
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The number of mitochondria per cell varies substantially from cell line to cell line. For example, human HeLa cells contain at least twice as many mitochondria as smaller mouse L cells. This protocol starts with a washed cell pellet of 1-2 mL derived from ∼10⁹ cells grown in culture. The cells are swollen in a hypotonic buffer and ruptured with a Dounce or Potter-Elvehjem homogenizer using a ti...
متن کاملisolation of adipose tissue stem cells with organ culture method
background isolation of adipose tissue mesenchymal stem cells (mscs) with current enzymatic methods has some limitations. for example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of mscs. also, it is accompanied with the increased risk of culture contaminations because of several handling steps.in this ...
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ژورنال
عنوان ژورنال: HortScience
سال: 2006
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.41.4.1024c